Cynandione A induces adipogenesis and beige adipocyte-related phenotype in 3T3-L1 cells. (October 2020)

• Record Type: Journal Article
• Authors: Sawamoto, Atsushi; Kanazaki, Asami; Amakura, Yoshiaki; Yoshimura, Morio; Masumoto, Naoko; Uchiyama, Nahoko; Hakamatsuka, Takashi; Okuyama, Satoshi; Furukawa, Yoshiko; Nakajima, Mitsunari
• Is Part Of: Phytochemistry letters. Volume 39(2020)
• Page Start: 84
• Page End: 89
• Publication Date: 2020-10
• Subjects: Cynandione A -- Beige adipocyte -- Adipogenesis -- 3T3-L1 cells; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2020.07.011
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Cynandione A promoted adipogenic differentiation and lipid accumulation in 3T3-L1 cells. Cynandione A up-regulated the expression of beige adipocyte-related genes in 3T3-L1 cells. Cynandione A up-regulated the expression of mitochondria-related genes and increased mitochondrial mass. Abstract: Cynandione A (CA) is a major ingredient of Cynanchum wilfordii . Here, we report a new function of CA in adipogenesis in 3T3-L1 cells, a preadipocyte cell line. During adipogenic differentiation in 3T3-L1 cells, CA boosted the process by enhancing the expression of key adipogenic transcription factors ( Pparg and C/ebpa ), brown adipocyte-related genes ( Prdm16, Pgc-1a, Cidea, and Ucp1 ), and beige adipocyte-related genes ( Tbx1 and Cited1 ). Additionally, CA increased mitochondrial mass and expression levels of mitochondria-related genes ( Sirt3 and Tfam ) in the cells. These results suggest that CA induces adipogenesis and beige adipocyte-related phenotype in adipocyte lineage cells.