A simple and an easy-to-synthesize turn-on fluorescent probe for rapid detection of Zn2+ and its application in bioimaging. Issue 46 (12th November 2020)

• Record Type: Journal Article
• Authors: Jiao, Shu-Yan; Kong, Lu-Ming; Liu, Guo-Qun; Jia, Xu; Tian, Jia; Liu, Ya-Ge; Zhang, Liu-Xue; Zhang, Wang-Xi; Li, Ying-Hua; Huang, Zeng
• Is Part Of: Tetrahedron letters. Volume 61:Issue 46(2020)
• Publication Date: 2020-11-12
• Subjects: Fluorescent probe -- Zn2+ -- Reversibility -- DFT calculations -- Cell imaging; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2020.152507
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Highlights: A novel fluorescent probe 1 for rapid detection of Zn 2+ was developed. The structure and the synthetic route of probe 1 were simple. Probe 1 can discriminate Zn 2+ from Cd 2+ and other cations. Probe 1 can be used for determination of Zn 2+ ion in living cells. Abstract: A simple novel and an easy-to-synthesize fluorescent probe 1 for detection of Zn 2+ based on 1-(4-amino-2-hydroxyphenyl)ethan-1-one was developed. Probe 1 exhibited an effectively selective and sensitive recognition towards Zn 2+ over Cd 2+ and other cations. The Job's plot and HRMS experiments reveal the formation of 1:1 binding stoichiometry between probe 1 and Zn 2+ . The association constant ( K ) of probe 1 with Zn 2+ was calculated to be 7.998 × 10 4 M −1 . The detection limit of probe 1 towards Zn 2+ was calculated at very low concentration up to 46 nM. The perfect reversibility of probe 1 for sensing Zn 2+ was also demonstrated. Detection mechanism of Zn 2+ ion by probe 1 was suggested through the analytical tools like UV–vis, FESEM, TEM, 1 H NMR titration and the DFT calculations experiments. More importantly, the fluorescence imaging in C6 cells suggested that simple sensor had great potential in the application of biological imaging.