A comparatively study of menaquinone-7 isolated from Cheonggukjang with vitamin K1 and menaquinone-4 on osteoblastic cells differentiation and mineralization. (September 2019)

• Record Type: Journal Article
• Authors: Wu, Wei-Jie; Gao, Haiyan; Jin, Jong-Sik; Ahn, Byung-Yong
• Is Part Of: Food and chemical toxicology. Volume 131(2019)
• Publication Date: 2019-09
• Subjects: Menaquinone -- Proliferation -- Osteoblasts -- OPG/RANKL -- Vitamin K; ALP alkaline phosphatase -- α-MEM α-minimum essential medium -- cOC γ-carboxylated osteocalcin -- ESI electrospray ionization -- FBS fetal bovine serum -- Gla γ-carboxyglutamic acid -- MK-4 menaquinone-4 -- MK-7 menaquinone-7 -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5diphenyl tetrazolium bromide -- OPG osteoprotegerin -- RANKL receptor activator of nuclear factor-kB ligand -- ucOC under-carboxylated osteocalcin; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.05.048
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The effect of menaquinone-7 isolated from cheonggukjang was comparatively investigated with vitamin K1 and menaquinone-4 on cell differentiation and mineralization of the osteoblastic cell line MC3T3-E1. Results indicated that all vitamin K species significantly increased MC3T3-E1 cell proliferation, cellular alkaline phosphatase activity, osteocalcin synthesis, and calcium deposition in a dose-dependent manner. Menaquinone-4 and menaquinone-7 had more potent effects on calcium deposition than vitamin K1, and their effects were only partly reduced by warfarin (γ-carboxylation inhibitor) treatment, while warfarin abolished the induction activity of vitamin K1 on calcification. This suggests that vitamin K1 and K2 (menaquinone-4 & menaquinone-7) may have different mechanisms in stimulating osteoblast mineralization. In addition, the mRNA expression ratio of osteoprotegerin and the receptor activator of nuclear factor-kB ligand was also dramatically increased by treatment with vitamin K1 (62%), menaquinone-4 (247%), and menaquinone-7 (329%), suggesting that vitamin K may suppress the formation of osteoclast by up-regulating the ratio of osteoprotegerin/receptor activator of nuclear factor-kB ligand in osteoblasts. These results provide compelling evidence that vitamin K1, menaquinone-4, and menaquinone-7 all can promote bone health, which might be associated with elevations in the osteoprotegerin/receptor activator of nuclear factor-kB ligand ratio. Graphical abstract: Image 1 Highlights: Vitamin K species significantly increased osteoblast differentiation and mineralization. Vitamin K1 and K2 have different mechanisms in stimulating osteoblast mineralization. Vitamin K may suppress osteoclast formation by up-regulating the ratio of OPG/RANKL in osteoblasts. Vitamin K promote bone regeneration in cultured osteoblastic MC3T3-E1 cells.