Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata. (November 2020)

• Record Type: Journal Article
• Authors: Barreto, A.; Carvalho, A.; Silva, D.; Pinto, E.; Almeida, A.; Paíga, P.; Correira-Sá, L.; Delerue-Matos, C.; Trindade, T.; Soares, A.M.V.M.; Hylland, K.; Loureiro, S.; Oliveira, M.
• Is Part Of: Marine pollution bulletin. Volume 160(2020)
• Publication Date: 2020-11
• Subjects: Fish liver culture -- Gilthead seabream -- Nanotoxicology -- Ionic gold -- Oxidative stress -- DNA integrity; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2020.111665
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: In vitro methods have gained rising importance in ecotoxicology due to ethical concerns. The aim of this study was to assess the single and combined in vitro effects of gold, as nanoparticle (AuNPs) and ionic (Au + ) form, and the pharmaceutical gemfibrozil (GEM). Sparus aurata liver organ culture was exposed to gold (4 to 7200 μg·L −1 ), GEM (1.5 to 15, 000 μg·L −1 ) and combination 80 μg·L −1 gold +150 μg·L −1 GEM for 24 h. Endpoints related with antioxidant status, peroxidative/genetic damage were assessed. AuNPs caused more effects than Au +, increasing catalase and glutathione reductase activities and damaging DNA and cellular membranes. Effects were dependent on AuNPs size, coating and concentration. GEM damaged DNA at an environmentally relevant concentration, 1.5 μg·L −1 . Overall, the effects of the combined exposures were higher than the predicted, based on single exposures. This study showed that liver culture can be a useful model to study contaminants effects. Highlights: In vitro, gold nanoparticles (AuNPs) damaged fish liver DNA and cellular membranes. Gemfibrozil (GEM) caused DNA damage at 1.5 μg·L −1 . Overall, effects of AuNPs+GEM were higher than predicted, based on single exposures. Liver organ culture proved sensitive and a valuable in vitro model.