Comparative study on enzymatic digestibility of acid-pretreated poplar and larch based on a comprehensive analysis of the lignin-derived recalcitrance. (January 2021)

• Record Type: Journal Article
• Authors: Jia, Yuan; Yang, Chundong; Shen, Buzhen; Ling, Zhe; Huang, Caoxing; Li, Xin; Lai, Chenhuan; Yong, Qiang
• Is Part Of: Bioresource technology. Volume 319(2021)
• Publication Date: 2021-01
• Subjects: Acid pretreatment -- Poplar -- Larch -- Enzymatic hydrolysis -- Lignin inhibition; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124225
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Enzymatic digestibility of pretreated poplar was better than the equivalent larch. Lignin fractions from AL showed the stronger inhibition than those from AP. Lignin distribution influenced the inhibition of enzyme non-productive binding. Lignin physical blocking played a more significant role in limiting AL hydrolysis. Abstract: Enzymatic digestibility of an acid-pretreated poplar (AP, 42.9%) was superior to that of a similarly acid-pretreated larch (AL, 12.5%). Effects of lignin-related recalcitrance on enzymatic hydrolysis were comprehensively investigated by disrupting the two predominant lignin fractions present in acid-pretreated material (extractable lignin and bulk lignin). Lignin removal and bovine serum albumin (BSA) addition were performed to estimate the relative contributions of lignin towards physical blocking and enzyme binding on enzymatic hydrolysis. The lignin physical blocking played a more significant role in limiting the enzymatic hydrolysis of AL. BSA addition improved enzymatic hydrolysis of AP more significantly than AL. Moreover, the effects of lignin embedded in the lignocellulosic matrix on enzyme non-productive binding were compared with the isolated lignin. It indicated that the lignin distribution would influence the lignin effects on enzyme non-productive binding during enzymatic hydrolysis. Results will give insights towards improvement of enzymatic hydrolysis on acid-pretreated woody biomass.