Nanosecond pulsed electric field processing of microalgae based biorefineries governs growth promotion or selective inactivation based on underlying microbial ecosystems. (January 2021)

• Record Type: Journal Article
• Authors: Haberkorn, Iris; Buchmann, Leandro; Häusermann, Iris; Mathys, Alexander
• Is Part Of: Bioresource technology. Volume 319(2021)
• Publication Date: 2021-01
• Subjects: Microalgae -- Nanosecond pulsed electric field -- Flow cytometry -- Growth stimulation -- Selective inactivation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124173
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Nanosecond pulsed electric field processing to foster single-cell biorefineries. Flow cytometry and advanced data analysis to monitor population dynamics. Phenotypic fingerprinting of prokaryotic communities for diversity analysis. Prokaryotic diversity governs growth stimulation or selective inactivation. Abstract: Nanosecond pulsed electric field treatment (nsPEF) is a technology-driven, resource-efficient approach fostering microalgae biorefineries for transforming them into economically viable scenarios. A processing window of 100 ns, 7 Hz, and 10 kV cm −1 significantly leveraged phototrophic Chlorella vulgaris and bacterial counts up to + 50.1 ± 12.2% and + 77.0 ± 37.4%, respectively (n = 4; p < 0.05) in non-axenic cultures. Applying the same processing window decreased C. vulgaris (−17.1 ± 13.8%) and prokaryotic (−82.7 ± 14.6%) counts owing to alterations in the prokaryotic community diversity. Principle coordinate analysis of prokaryotic phenotypic fingerprints indicated that phenotype or metabolism related diversity changes in the prokaryotic community affected the treatment outcome. The study fosters the upsurge of industrial-scale nsPEF realization and the economic viability of microalgae biorefineries through improved process understanding and thus control. It perpetuates nsPEF applicability for microalgae feedstock production and several other applications within single-cell biorefineries in the bio-based domain.