Time Resolved Spectroscopy Using Boxcar Integrator. (July 2019)

• Record Type: Journal Article
• Authors: Perangin-angin, Bisman; Tarigan, Kerista; Sihombing, Poltak
• Is Part Of: Journal of physics. Volume 1230(2019)
• Publication Date: 2019-07
• Subjects: Physics -- Congresses; 530.5
• DOI: 10.1088/1742-6596/1230/1/012034
• Languages: English
• ISSNs: 1742-6588

Abstract

Abstract: An observation of fluorescence decay signals of condensed-state molecular samples in the time domain has been proved which is very useful for its identification and analysis. To minimize the noise effect in the fluorescence signals, a double-gated boxcar integrator is designed and incorporated into a spectrometer. The design concept and the working principle of the develop spectrometer are presented along with the detailed subsystems description. The performance of the spectrometer is tested with a well-known sample (rhodamine 6G) and the results are dually analyzed. The application of the spectrometer is used to measure the fluorescence spectrum and mean lifetime of an E. Coli bacteria sample. The lifetime of the E.Coli is about 10 ns which is obtained at peak wavelength of 506 nm.