Genetic variability of Prochilodus lineatus in artificial and semi-natural reproduction. Issue 2 (3rd April 2018)

• Record Type: Journal Article
• Authors: Souza, Felipe Pinheiro de; Castro, Pedro Luiz de; Goes, Elenice Souza dos Reis; Ribeiro, Ricardo Pereira; Santos, Silvio Carlos Alves dos; Lima, Ed Christian Suzuki de; Murari, Pamela Juliana Furlan; Lopera-Barrero, Nelson Maurício
• Is Part Of: Italian journal of animal science. Volume 17:Issue 2(2018)
• Page Start: 321
• Page End: 325
• Publication Date: 2018-04-03
• Subjects: Broodstock -- conservation -- Curimba -- microsatellite -- restocking programs; Animal culture -- Periodicals; Livestock -- Italy -- Periodicals; Veterinary medicine -- Italy -- Periodicals; Animal culture; Livestock; Veterinary medicine; Italy; Periodicals; Periodicals; 636.005
• DOI: 10.1080/1828051X.2017.1365312
• Languages: English
• ISSNs: 1828-051X

Abstract

Abstract: Prochilodus lineatus is a South American fish of great economic and social importance that is impacted by high fishing pressure. Restocking programmes have tried to be effective in recovering natural populations; however, genetic and reproductive methodologies are necessary to make feasible the survival of released fingerlings. The genetic variability of P. lineatus subjected to semi-natural and artificial reproduction was evaluated. Caudal fin samples from 25 breeders subjected to an artificial reproduction were collected at different sex ratios (1♂:1♀ and 2♂:1♀), and 138 fingerlings originated from these spawning. Similarly, samples were collected from 10 breeders (1♂:1♀) subjected to a semi-natural reproduction, and 70 fingerlings were generated from this spawning. Ten microsatellite loci were evaluated, producing 142 alleles. High interpopulation variability for broodstock and fingerlings was observed in both reproductive systems. A deficit of heterozygotes through the inbreeding coefficient (Fis) was obtained. Moreover, a deviation in the Hardy–Weinberg equilibrium of the fingerlings in both systems was detected. Fst values indicated a moderate genetic differentiation in 1:1 artificial reproduction system and low differentiation in 2:1 artificial and 1:1 seminatural reproduction system. Despite the high genetic variability, genetics analyses in the next generations of fingerlings will be necessary considering the likelihood of a decrease in genetic variability.