A perspective view on the nanomotion detection of living organisms and its features. Issue 12 (30th March 2020)

• Record Type: Journal Article
• Authors: Venturelli, Leonardo; Kohler, Anne‐Céline; Stupar, Petar; Villalba, Maria I.; Kalauzi, Aleksandar; Radotic, Ksenija; Bertacchi, Massimiliano; Dinarelli, Simone; Girasole, Marco; Pešić, Milica; Banković, Jasna; Vela, Maria E.; Yantorno, Osvaldo; Willaert, Ronnie; Dietler, Giovanni; Longo, Giovanni; Kasas, Sandor
• Is Part Of: Journal of molecular recognition. Volume 33:Issue 12(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-30
• Subjects: AFM -- antibiotic susceptibility test -- cellular metabolism -- nanobiosensors -- nanomechanical sensors -- nanomotion -- single cell investigation; Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2849
• Languages: English
• ISSNs: 0952-3499

Abstract

Abstract: The insurgence of newly arising, rapidly developing health threats, such as drug‐resistant bacteria and cancers, is one of the most urgent public‐health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro‐fabricated cantilever and by monitoring its displacements with an atomic force microscope‐based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life‐threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.