Transcriptional meta‐analysis of regulatory B cells. Issue 11 (23rd June 2020)

• Record Type: Journal Article
• Authors: Dubois, Florian; Limou, Sophie; Chesneau, Mélanie; Degauque, Nicolas; Brouard, Sophie; Danger, Richard
• Is Part Of: European journal of immunology. Volume 50:Issue 11(2020)
• Page Start: 1757
• Page End: 1769
• Publication Date: 2020-06-23
• Subjects: meta‐analysis -- gene expression -- B cells -- Bregs -- granzyme B; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201948489
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract: Regulatory B cells (Bregs) have the ability to regulate inflammation in various pathological situations, making them key players in immune regulation. Several mechanisms have been described and we recently identified a GZMB expressing Breg population in kidney transplanted patients who tolerate a kidney graft. To further investigate their biology and mechanisms, we conducted a transcriptomic analysis by RNAseq of these cells and we performed the first weighted meta‐analysis of publicly available transcriptomic data from published Breg studies both in humans and mice. We identified two distinct and unique transcriptional signatures of 126 and 93 genes, respectively, associated with these Bregs. While we highlighted genes coding for proteins with potent involvement in regulatory functions, proliferation, and coding for transcription factors, the comparison between humans and mice did not allow identifying a common pattern. Thus, our results suggest distinct species‐restricted Breg transcriptional signatures in humans and mice. Abstract : Through a gene expression meta‐analysis comparing different Bregs and non‐regulatory B cell populations, we evidenced two distinct transcriptional signatures of 126 and 93 genes associated with human and mouse Bregs, respectively, without common pattern. These results will help understanding Bregs biology and facilitate their studies.