Tissue‐resident mucosal‐associated invariant T (MAIT) cells in the human kidney represent a functionally distinct subset. Issue 11 (6th August 2020)

• Record Type: Journal Article
• Authors: Terpstra, Matty L.; Remmerswaal, Ester B.M.; van der Bom‐Baylon, Nelly D.; Sinnige, Marjan J.; Kers, Jesper; van Aalderen, Michiel C.; Geerlings, Suzanne E.; Bemelman, Frederike J.
• Is Part Of: European journal of immunology. Volume 50:Issue 11(2020)
• Page Start: 1783
• Page End: 1797
• Publication Date: 2020-08-06
• Subjects: MAIT cells -- renal transplantation -- tissue‐residency; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.202048644
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract: Mucosal‐associated invariant T (MAIT) cells are innate‐like T‐cells that recognize bacterial riboflavin metabolites. They are present in human blood but are abundant at barrier sites, including the liver, lungs, and kidneys, where they possess a CD69 + /CD103 +/− tissue‐resident phenotype. In renal tissue, MAIT cells likely defend against the ascending uropathogens responsible for urinary tract infections (UTIs), which are common, especially among renal transplant recipients (RTRs). Nevertheless, the functional role for MAIT cells in renal tissue and the influence of renal transplantation on MAIT cells remains unclear. Using multiparameter flow cytometry and the MR1‐tetramer, we characterized MAIT cell phenotype and function in healthy renal tissue (n = 6), renal transplants explanted after allograft failure (n = 14) and in blood from healthy controls (n = 20) and RTRs before and 1‐year after transplantation (n = 21). MAIT cells in renal tissue constitute a distinct CD69 + CD103 +/− population that displays typical phenotypic features of tissue‐resident T‐cells and is skewed toward IL‐2, GM‐CSF, and IL‐17A production upon stimulation. The circulating MAIT cell population was not decreased in number in RTRs pre‐ or post‐transplantation. Tissue‐resident MAIT cells in the kidney represent a functionally distinct population. This shows how MAIT cells in the kidney may be involved in the protection against microorganisms. Abstract : MAIT cells, innate‐like T‐cells involved in the antibacterial response, have recently been identified for the first time in renal tissue. Our results show that MAIT cells in the kidney express a distinct tissue‐resident phenotype and possess specialized local memory functions such as the production of immune response enhancing cytokines.