A rapid white blood cell classification system based on multimode imaging technology. Issue 11 (2nd September 2020)

• Record Type: Journal Article
• Authors: Lv, Meng; Zhao, Xi; Chen, Feng; Yu, Ming; Li, Chao; Sun, Jinggong
• Is Part Of: Journal of biophotonics. Volume 13:Issue 11(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-02
• Subjects: BP neural network -- imaging systems -- microfluidic chip -- multimode imaging; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202000197
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: In order to simplify the complexity of white blood cell classification in existing point‐of‐care testing (POCT) testing equipment, a white blood cell classification detection system based on microfluidic and multimode imaging was constructed. Microfluidic chip was used in the system. A multimodal optical imaging system based on the characteristics of blood samples was designed to obtain eigenvalue extraction of cells. Afterward, a BP neural network model was constructed to realize automatic classification of white blood cells. Finally, 80 human blood samples were classified and detected by this system and compared with the results of Sysmex XE‐5000. The consistency correlation coefficients of white blood cells, lymphocytes, monocytes, neutrophils and eosinophils are 1.038, 0.907, 0.549, 0.922 and 1.028, respectively, and the CV values of the four types of white blood cells in the stability test were all below 10%. In this study, a white blood cell classification and detection system with small size, simple operation, fast single‐sample detection, high accuracy, and no maintenance is required. It will provide a solid technical support for the further development of POCT blood cell analysis equipment. Abstract : In order to simplify the complexity of the white blood cell classification method in the existing testing equipment, a white blood cell classification and detection system based on microfluidic chip technology and multimode imaging technology was constructed. It can realize the classification and detection of four types of white blood cells, and provides solid technical support for the further development of POCT blood cell detection equipment.