Determination of berberine in Rhizoma coptidis using a β-cyclodextrin-sensitized fluorescence method. Issue 66 (4th November 2020)

• Record Type: Journal Article
• Authors: Zheng, Yan-Hui; Li, Wen-Hong; Chen, Peng; Zhou, Ying; Lu, Wenbo; Ma, Zi-Chuan
• Is Part Of: RSC advances. Volume 10:Issue 66(2020)
• Page Start: 40136
• Page End: 40141
• Publication Date: 2020-11-04
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra07573f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : In this work, a method for the determination of berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology is established. Abstract : In this work, a method for the determination of berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology is established. Berberine is the main extract of Rhizoma coptidis, a medicinal material, which causes an envelope reaction with β-cyclodextrin to generate fluorescence sensitization. In the environment of its own aqueous extract, with 0.0065 mol L −1 of β-cyclodextrin, a fluorescence excitation wavelength ( λ ex ) of 345 nm and an emission wavelength ( λ em ) of 540 nm were selected to avoid interference from other distractors. The fluorescent sensor for the detection of berberine exhibits a low limit of detection (3.59 × 10 −9 mol L −1 ) and a wide linear range from 2.7 × 10 −7 mol L −1 to 2.7 × 10 −6 mol L −1 . Our sensor can be also used to detect berberine in real medicinal materials. The content of berberine in Rhizoma coptidis medicinal material was found to be 7.60% using this method with an average recovery rate of 99.5%. The result obtained by thin-layer chromatography with fluorescence detection was 7.61%, which is consistent with the result from the β-cyclodextrin sensitized fluorescence method. This method is simple and environmentally friendly with high sensitivity and good selectivity and gives reliable results, which is promising for practical application.