Amplified piezoelectrically actuated on-chip flow switching for a rapid and stable microfluidic fluorescence activated cell sorter. Issue 66 (5th November 2020)

• Record Type: Journal Article
• Authors: Cai, Kunpeng; Mankar, Shruti; Maslova, Anastasia; Ajiri, Taiga; Yotoriyama, Tasuku
• Is Part Of: RSC advances. Volume 10:Issue 66(2020)
• Page Start: 40395
• Page End: 40405
• Publication Date: 2020-11-05
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra04919k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : With the potential to avoid cross-contamination, eliminate bio-aerosols, and minimize device footprints, microfluidic fluorescence-activated cell sorting (μ-FACS) devices could become the platform for the next generation cell sorter. Abstract : With the potential to avoid cross-contamination, eliminate bio-aerosols, and minimize device footprints, microfluidic fluorescence-activated cell sorting (μ-FACS) devices could become the platform for the next generation cell sorter. Here, we report an on-chip flow switching based μ-FACS mechanism with piezoelectric actuation as a fast and robust sorting solution. A microfluidic chip with bifurcate configuration and displacement amplified piezoelectric microvalves has been developed to build the μ-FACS system. Rare fluorescent microparticles of different sizes have been significantly enriched from a purity of ∼0.5% to more than 90%. An enrichment of 150-fold from ∼0.6% to ∼91% has also been confirmed for fluorescently labeled MCF-7 breast cancer cells from Jurkat cells, while viability after sorting was maintained. Taking advantage of its simple structure, low cost, fast response, and reliable flow regulation, the proposed μ-FACS system delivers a new option that can meet the requirements of sorting performance, target selectivity, device lifetime, and cost-effectiveness of implementation.