Multifunctional, histidine-tagged polymers: antibody conjugation and signal amplification. Issue 89 (21st October 2020)

• Record Type: Journal Article
• Authors: Zhang, Yichuan; Gambardella, Alessia; Üçüncü, Muhammed; Geng, Jin; Clavadetscher, Jessica; Bradley, Mark; Lilienkampf, Annamaria
• Is Part Of: Chemical communications. Volume 56:Issue 89(2020)
• Page Start: 13856
• Page End: 13859
• Publication Date: 2020-10-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc04591h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A polymer scaffold, with multiple reactive centres, was synthesised by RAFT polymerisation and conjugated to the antibody herceptin. A hexahistidine RAFT agent enabled simple purification of polymer–protein conjugates. Abstract : A polymer scaffold, with multiple reactive centres, was synthesised by RAFT polymerisation and conjugated to the antibody herceptin. A hexahistidine RAFT agent enabled the rapid and simple purification of polymer–protein conjugates, while the tetrazine conjugation strategy allows myriad cargos to be attached and amplified.