Role of the regulatory genes SEF1, VMA1 and SFU1 in riboflavin synthesis in the flavinogenic yeast Candida famata (Candida flareri). Issue 9 (2nd July 2020)

• Record Type: Journal Article
• Authors: Andreieva, Yuliia; Petrovska, Yana; Lyzak, Oleksii; Liu, Wen; Kang, Yingqian; Dmytruk, Kostyantyn; Sibirny, Andriy
• Other Names: Erten Huseyin guestEditor.; Sibirny Andriy guestEditor.
• Is Part Of: Yeast. Volume 37:Issue 9-10(2020:Sep./Oct.)
• Page Start: 497
• Page End: 504
• Publication Date: 2020-07-02
• Subjects: Candida famata -- riboflavin -- SEF1 -- SFU1 -- VMA1 -- yeast; Yeast -- Periodicals; Yeasts -- Periodicals; Yeasts -- genetics -- Periodicals; Electronic journals; 547
• DOI: 10.1002/yea.3503
• Languages: English
• ISSNs: 0749-503X

Abstract

Abstract: Riboflavin or vitamin B2 is an essential dietary component for humans and animals that is the precursor of flavin coenzymes flavin mononucleotide and flavin adenine dinucleotide involved in numerous enzymatic reactions. The flavinogenic yeast Candida famata overproduces riboflavin under iron starvation; however, regulation of this process is poorly understood. Regulatory gene SEF1 encoding transcription activator has been identified. Its deletion blocks yeast ability to overproduce riboflavin under iron starvation. It was shown here that the SEF1 promoters from other flavinogenic ( Candida albicans ) and non‐flavinogenic ( Candida tropicalis ) yeasts fused with the open reading frame (ORF) of SEF1 gene from C. famata are able to restore riboflavin oversynthesis in sef1Δ mutants. It is known that in the pathogenic flavinogenic yeast C. albicans, Sfu1 (GATA‐type transcription factor) represses SEF1. Here, we found that deletion of SFU1 gene in wild‐type C. famata leads to riboflavin oversynthesis. Moreover, it was shown that disruption of VMA1 gene (coding for vacuolar ATPase subunit A) also results in riboflavin oversynthesis in C. famata .