Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: Relevance for male fertility. (3rd February 2015)

• Record Type: Journal Article
• Authors: Dias, Tânia R.; Alves, Marco G.; Bernardino, Raquel L.; Martins, Ana D.; Moreira, Ana C.; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Silva, Branca M.; Oliveira, Pedro F.
• Is Part Of: Toxicology. Volume 328(2015)
• Page Start: 12
• Page End: 20
• Publication Date: 2015-02-03
• Subjects: 4-HNE 4-hydroxynonenal -- DNP 2, 4-dinitrophenyl -- DNPH 2, 4-dinitrophenylhydrazine -- FBP fructose-1, 6-biphosphate -- FRAP ferric reducing antioxidant power -- FSH follicle-stimulating hormone -- GLUT1 glucose transporter 1 -- GLUT3 glucose transporter 3 -- hSCs human Sertoli cells -- LDH lactate dehydrogenase -- MCT4 monocarboxylate transporter 4 -- OS oxidative stress -- PBS phosphatebuffered saline -- PFK1 phosphofructokinase 1 -- PVDF polyvinylidenedifluoride -- ROS reactiveoxygenspecies -- SC Sertoli cell -- TBS Tris–buffered saline -- TFA trifuoroaceticacid -- TPTZ 2, 4, 6-tripyridyl-s-triazine; Caffeine -- Sertoli cell -- Spermatogenesis -- Cell metabolism -- Lactate -- Male fertility; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2014.12.003
• Languages: English
• ISSNs: 0300-483X

Abstract

Highlights: Caffeine stimulates lactate production by human Sertoli cells. Lactate dehydrogenase activity was enhanced to sustain lactate production. Expression of glucose transporters was increased by caffeine in human Sertoli cells. Caffeine reduced protein oxidation and lipid peroxidation in human Sertoli cells. Moderate consumption of caffeine appears to be safe to male reproductive health. Abstract: Caffeine is a widely consumed substance present in several beverages. There is an increasing consumption of energetic drinks, rich in caffeine, among young individuals in reproductive age. Caffeine has been described as a modulator of cellular metabolism. Hence, we hypothesized that it alters human Sertoli cells (hSCs) metabolism and oxidative profile, which are essential for spermatogenesis. For that purpose, hSCs were cultured with increasing doses of caffeine (5, 50, 500 μM). Caffeine at the lowest concentrations (5 and 50 μM) stimulated lactate production, but only hSCs exposed to 50 μM showed increased expression of glucose transporters (GLUTs). At the highest concentration (500 μM), caffeine stimulated LDH activity to sustain lactate production. Notably, the antioxidant capacity of hSCs decreased in a dose-dependent manner and SCs exposed to 500 μM caffeine presented a pro-oxidant potential, with a concurrent increase of protein oxidative damage. Hence, moderate consumption of caffeine appears to be safe to male reproductive health since it stimulates lactate production by SCs, which can promote germ cells survival. Nevertheless, caution should be taken by heavy consumers of energetic beverages and food supplemented with caffeine to avoid deleterious effects in hSCs functioning and thus, abnormal spermatogenesis.