Gamisasangja-tang suppresses pruritus and atopic skin inflammation in the NC/Nga murine model of atopic dermatitis. (13th May 2015)

• Record Type: Journal Article
• Authors: Park, Bo-Kyung; Park, Yang-Chun; Jung, In Chul; Kim, Seung-Hyung; Choi, Jeong June; Do, Moonho; Kim, Sun Yeou; Jin, Mirim
• Is Part Of: Journal of ethnopharmacology. Volume 165(2015)
• Page Start: 54
• Page End: 60
• Publication Date: 2015-05-13
• Subjects: ANOVA analysis of variance -- CCR3 chemokine (C-C motif) receptor 3 -- CD cluster of differentiation -- DNCB 2, 4-dinitrochlorobenzene -- DNFB 2, 4-dinitrofluorobenzene -- FACS fluorescence-activated cell sorting -- FITC fluorescein isothiocyanate -- GAPDH glyceraldehyde-3 phosphate dehydrogenase -- ELISA enzyme-linked immunosorbent assay -- FcεRIα Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide -- GST Gamisasangja-tang -- Dex dexamethasone -- IgE immunoglobulin E -- IL interleukin -- NC NC/Nga -- PBS phosphate buffered saline -- PE phycoerythrin -- PerCP peridinin chlorophyll protein complex -- PCR polymerase chain reaction -- ST sasangja-tang -- TARC thymus and activation regulated chemokine -- Th2 T-helper cell type 2; Chlorogenic acid (PubChem CID: 1794427) -- Vitextin (PubChem CID: 5280441) -- Decursin (PubChem CID: 442126) -- Torilin (PubChem CID:6450226); Gamisasangja-tang (GST) -- Pruritus -- Atopic skin inflammation -- IL-31 -- NC/Nga murine model; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.02.040
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Gamisasangja-tang (GST) is a traditional herbal formula prescribed for patients with intractable pruritus in association with various inflammatory skin diseases. To evaluate the effects of GST on pruritic skin inflammation and investigate its cellular and molecular mechanisms. Materials and methods: We orally administered GST to NC/Nga (NC) mice, an animal model of atopic dermatitis. Scratching frequency and the dermatitis index were evaluated, and histological examination was performed using hematoxylin and eosin and toluidine blue staining. The levels of interleukin (IL)-31 and T-helper cell type 2 (TH 2) cytokines were determined in both the dorsal skin and cultured splenocytes by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The serum levels of chemokines and immunoglobulin E (IgE) were determined by ELISA. Changes in the inflammatory cell population were analyzed by a hemocytometer. Results: GST significantly lowered scratching frequency and inhibited increases in dermatitis index, thickness of epidermis/dermis and infiltration of chemokine (C-C motif) receptor 3 (CCR3) + and cluster of differentiation (CD)117 + /FcεRI α (Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide) + cells in atopic skin. Both IL- 31 mRNA expression and production were significantly reduced by GST, which was accomrease in the levels of IL-4, IL-5, and IL-13 . Further, GST treatment suppressed the secretion of eotaxin, TARC (thymus and activation-regulated chemokine), IgE, and increases in the number of basophils and eosinophils in the blood. Conclusion: GST may have potential as an effective treatment for pruritic skin disease such as atopic dermatitis. Graphical abstract: