Novel anti-angiogenic effects of aromatic-turmerone, essential oil isolated from spice turmeric. (May 2015)

• Record Type: Journal Article
• Authors: Yue, Grace Gar-Lee; Kwok, Hin-Fai; Lee, Julia Kin-Ming; Jiang, Lei; Chan, Kar-Man; Cheng, Ling; Wong, Eric Chun-Wai; Leung, Ping-Chung; Fung, Kwok-Pui; Lau, Clara Bik-San
• Is Part Of: Journal of functional foods. Volume 15(2015)
• Page Start: 243
• Page End: 253
• Publication Date: 2015-05
• Subjects: bFGF basic fibroblast growth factor -- FBS fetal bovine serum -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- HMEC-1 human microvascular endothelial cells -- PBS phosphate-buffered saline -- VEGF vascular endothelial growth factor; Aromatic-turmerone -- Angiogenesis -- VEGF -- Curcuma longa -- Endothelial cells -- Zebrafish; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.03.030
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: In vitro anti-angiogenic activities of aromatic-turmerone in human endothelial cells. In vivo anti-angiogenic activities of aromatic-turmerone in zebrafish embryos and matrigel plug mouse model. This is possibly the first report on the anti-angiogenic activities of aromatic-turmerone. Provided scientific insight into the development of such a component of turmeric essential oil to be an anti-angiogenic agent. Abstract: Angiogenesis is essential for tumor growth and there is a continuing need for exploring new anti-angiogenic agents from natural products including herbs. Aromatic (Ar)-turmerone isolated from the rhizome of Curcuma longa Linn. (Turmeric) exhibits anti-tumor and immunomodulatory activities. In this study, the anti-angiogenic effects of Ar-turmerone were evaluated in human microvascular endothelial cells, zebrafish and Matrigel plugs mouse models. The data obtained indicate that Ar-turmerone treatment significantly inhibits the proliferation, tube formation and motility of HMEC-1 cells at non-cytotoxic concentrations (4.6–9.2 µM, p < 0.05). The mRNA expressions of metalloproteinase-2 and -9 as well as adhesion molecules could be down-regulated by Ar-turmerone at 18.4 µM ( p < 0.05). In zebrafish model, the new blood vessel growth in embryos was significantly blocked by Ar-turmerone treatment (12.5–25 µg/mL medium). The bFGF-induced blood vessel formation in Matrigel plugs in C57BL/6 mice was suppressed by Ar-turmerone (25–50 µg/mL Matrigel). Thus, the in vitro and in vivo anti-angiogenic activities of Ar-turmerone were demonstrated for the first time. The findings suggest that such a component of turmeric essential oil has the potential to be further developed as an anti-angiogenic agent.