Allicin inhibits invasion and migration of breast cancer cells through the suppression of VCAM-1: Regulation of association between p65 and ER-α. (May 2015)

• Record Type: Journal Article
• Authors: Lee, Chung Gi; Lee, Hee-Weon; Kim, Byung-Oh; Rhee, Dong-Kwon; Pyo, Suhkneung
• Is Part Of: Journal of functional foods. Volume 15(2015)
• Page Start: 172
• Page End: 185
• Publication Date: 2015-05
• Subjects: Allicin -- Invasion -- Metastasis -- VCAM-1 -- ER-α; Allicin (CID: 65036) -- Penicillin (CID: 5904) -- Streptomycin (CID: 19649) -- HEPES (CID: 23831) -- MgCl2 (CID: 5360315) -- KCl (CID: 4873) -- DTT (CID: 446094) -- PMSF (CID: 4784) -- SDS (CID: 3423265) -- Glycerol (CID: 753); Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.03.017
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Allicin reduced tumor cell migration and invasion only in MCF-7 cells. Allicin is capable of inhibiting the expression of VCAM-1. Allicin treatment alters the expression of NF-κB and ER-α in TNF-α-stimulated MCF-7 cells. Allicin suppressed TNF-α-induced activation of ERK1/2. Abstract: Allicin, one of the major components of garlic, has wide-ranging health properties. However, the effects of allicin on migration and invasion of breast cancer cells remain unknown. Allicin (0.1–10 ng/ml) inhibited TNF-α-induced VCAM-1 protein expression in MCF-7, but not in MDA-MB-231. Additionally, allicin significantly reduced tumor cell migration and invasion only in MCF-7 cells. Our results also demonstrated that protein interaction between ER-α and p65 was down-regulated by TNF-α treatment, and this effect was reversed by allicin. Moreover, the inhibition of ER-α expression markedly enhanced TNF-α-induced migration and invasion of MCF-7 cells. Allicin strongly suppressed TNF-α-induced activation of ERK1/2. Collectively, these results suggest that allicin inhibited the TNF-α-mediated induction of VCAM-1 through blocking ERK1/2 and NF-κB signaling pathways and enhancing interaction between ER-α and p65, leading to the suppression of invasion and metastasis of MCF-7 cells. Therefore, allicin could be useful for preventing the advancement of breast cancer.