Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis. (17th October 2020)

• Record Type: Journal Article
• Authors: Yan, Xiaoteng; Yan, Zhuan; Xiong, Qingping; Liu, Gaoqin; Zhu, Juanjuan; Lu, Peirong
• Other Names: Faria Robson Xavier Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2020(2020)
• Publication Date: 2020-10-17
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2020/5175138
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinalis was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Then, we found that total polysaccharide content of APBOM was 94.37% with a sulfuric acid group of 1.68%, uronic acid content of 17.41%, and average molecular weight of 165.4 kDa. Finally, APBOM was confirmed to have significant antiangiogenic effects.