Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands. Issue 9 (3rd March 2016)

• Record Type: Journal Article
• Authors: Carvalho, Josué; Ferreira, João; Pereira, P.; Coutinho, E.; Guédin, Aurore; Nottelet, Pierre; Salgado, Gilmar F.; Mergny, Jean-Louis; Queiroz, J.A.; Sousa, F.; Cabrita, Eurico J.; Cruz, C.
• Is Part Of: Tetrahedron. Volume 72:Issue 9(2016)
• Page Start: 1229
• Page End: 1237
• Publication Date: 2016-03-03
• Subjects: G-quadruplex ligands -- Circular dichroism -- Thermal difference spectra -- FRET melting -- PCR-stop -- G4-FID; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2016.01.022
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: The topological study of novel quadruplex-forming DNA sequences found in immunoglobulin switch regions Sμ and Sγ3 is described along with the synthesis and characterization of naphthalene and quinoline-based ligands for the G-quadruplex binding and stabilization. Several biophysical and biochemical techniques are employed to assess the G-quadruplex formation and stabilization by the ligands. The results indicate that 58Sγ3 folds into a parallel-stranded G-quadruplex structure in K + buffer while 33Sμ folds into a hybrid-type G-quadruplex in the same buffer. The novel naphthalene and quinoline-based ligands bind and stabilize specifically a variety of G-quadruplexes. This study provides evidence of the importance of G-quadruplex structures to the biology of immunoglobulin class switch recombination. Graphical abstract: