Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: Effects on hydA expression. (30th October 2020)

• Record Type: Journal Article
• Authors: Moura, A.G.L.; Rabelo, C.A.B.S.; Okino, C.H.; Maintinguer, S.I.; Silva, E.L.; Varesche, M.B.A.
• Is Part Of: International journal of hydrogen energy. Volume 45:Number 53(2020)
• Page Start: 28447
• Page End: 28461
• Publication Date: 2020-10-30
• Subjects: Iron nanoparticles -- Nickel nanoparticles -- Dark fermentation -- Clostridium butyricum -- Response surface methodology; ANOVA Analyses of variance -- B:A Butyric acid and Acetic acid ratio -- CCT Tropical Cultures Collection -- CCD Central Composite Design -- cDNA Complementary DNA -- CN Copy Numbers -- COD Chemical Oxygen Demand -- Cq Quantification Cycles -- Ct Threshold Cycles -- E qPCR Reaction Efficiency -- Ehyd Efficiency of qPCR reaction with hyd gene -- EPfo Efficiency of qPCR reaction with Pfo gene -- EHPS Exponential Hydrogen Production Stage -- H2 Hydrogen -- hydA Hydrogenase Target Gene -- MFC Microbial Fuel Cell -- NPs Nanoparticles -- NTC No Template Control -- P Accumulated Hydrogen Production -- PB Plackett-Burman -- PCR Polymerase Chain Reaction -- qPCR real-time Polymerase Chain Reaction -- RCCD Rotational Central Composite Design -- RCM Reinforced Clostridial Medium -- RSM Response Surface Methodology -- RT Reverse Transcription -- HY Hydrogen yield -- VS Volatile Solidsl -- VFA Volatile Fatty Acids -- VSS Volatile Suspended Solids; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2020.07.161
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: In this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2 ) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe 0 /L and 20 mg Ni 0 /L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one. Graphical abstract: Image 1 Highlights: Design of experiments was applied for maximum HY achievement with C. butyricum . Higher H2 production (434 ± 13 mL) was achieved with 30 mg Fe 0 /L and 20 mg Ni 0 /L. NPs shifted metabolic pathway from acetic and butyric to valeric acid production. Pfo was the best reference gene for specific monitoring of C. butyricum hydA gene. HydA gene copy numbers with NPs was 40 folds higher as compared with control.