Bioinformatic analysis of eosinophil activity and its implications for model and target species. Issue 4 (16th April 2020)

• Record Type: Journal Article
• Authors: Jenvey, C.J.; Alenizi, D.; Almasi, F.; Cairns, C.; Holmes, A.; Sloan, S.; Stear, M.J.
• Is Part Of: Parasitology. Volume 147:Issue 4(2020)
• Page Start: 393
• Page End: 400
• Publication Date: 2020-04-16
• Subjects: Bioinformatics, -- CCR3, -- eosinophils, -- EPX, -- FcαRI, -- gastrointestinal nematodes, -- PRG3, -- ruminants; Parasitology -- Periodicals; Electronic journals; 616.96
• DOI: 10.1017/S0031182019001768
• Languages: English
• ISSNs: 0031-1820

Abstract

Abstract: Eosinophils are important immune cells that have been implicated in resistance to gastrointestinal nematode (GIN) infections in both naturally and experimentally infected sheep. Proteins of particular importance appear to be IgA-Fc alpha receptor (Fc α RI), C-C chemokine receptor type 3 (CCR3), proteoglycan 3 (PRG3, major basic protein 2) and EPX (eosinophil peroxidase). We used known human nucleotide sequences to search the ruminant genomes, followed by translation to protein and sequence alignments to visualize differences between sequences and species. Where a sequence was retrieved for cow, but not for sheep and goat, this was used additionally as a reference sequence. In this review, we show that eosinophil function varies among host species. Consequently, investigations into the mechanisms of ruminant immune responses to GIN should be conducted using the natural host. Specifically, we address differences in protein sequence and structure for eosinophil proteins.