An L012@PAni-PAAm hydrogel composite based-electrochemiluminescence biosensor for in situ detection of H2O2 released from cardiomyocytes. (10th September 2020)

• Record Type: Journal Article
• Authors: Guo, Xiaojin; Li, Yabei; Li, Yingchun; Ye, Zhaoyang; Zhang, Junjie; Zhu, Tong; Li, Fei
• Is Part Of: Electrochimica acta. Volume 354(2020)
• Publication Date: 2020-09-10
• Subjects: H2O2 detection -- electrochemiluminescence -- L012@PAni-PAAm hydrogel composite -- cardiomyocytes; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2020.136763
• Languages: English
• ISSNs: 0013-4686

Abstract

Research Highlights: l An L012@PAni-PAAm hydrogel composite was prepared on glassy carbon electrode and used as a novel ECL biosensor. l The L012@PAni-PAAm hydrogel composite-based ECL biosensor presents good stability, high selectivity, wide detection range (0.01–50 μM) and high detection sensitivity (limit of detection of 2.9 nM) of H2 O2 . l The ECL biosensor was sucessfully applied to in situ detect H2 O2 produced by cardiomyocytes. Abstract: Detection of hydrogen peroxide (H2 O2 ) released from cardiomyocytes is vital for pathological and physiological studies of cardiovascular diseases (CVD). Electrochemiluminescence (ECL) method has been widely utilized to monitor cellular H2 O2 owing to its advantages of high sensitivity and specificity. In this work, we proposed a novel ECL biosensor based on an L012@PAni-PAAm hydrogel composite for in situ monitoring H2 O2 produced from cardiomyocytes. Besides the decent porosity for cell adhesion, favorable biocompatibility for cell incubation and high permeability for H2 O2 diffusion, the L012@PAni-PAAm hydrogel composite exhibited good conductivity for triggering ECL reaction and high detection sensitivity of H2 O2 . The L012@PAni-PAAm hydrogel composite-based ECL biosensor showed low background signal, good stability, high selectivity, wide detection range (0.01–50 μM) and high detection sensitivity (limit of detection of 2.9 nM) of H2 O2 . Thus, it was successfully applied to in situ detect H2 O2 produced from cardiomyocytes. The ECL biosensor developed in our work can be used as a new highly sensitive biosensor for in situ detection of cellular H2 O2 for physiological and pathological studies. Graphic Abstract: A novel ECL biosensor based on L012@PAni-PAAm hydrogel composite modified GCE was developed and used to in situ monitor H2 O2 produced from cardiomyocytes. Image, graphical abstract