A Simple Blocking PCR‐Based Method for the Synthesis of High‐Copy dsDNA Tandem Repeats. Issue 43 (1st October 2020)

• Record Type: Journal Article
• Authors: Wang, Xin; Yang, Xuerui
• Is Part Of: Small. Volume 16:Issue 43(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-01
• Subjects: blocking PCR -- BPRE -- DNA hydrogel -- DNA tandem repeat -- high‐copy direct DNA repeat; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202003671
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: DNA tandem repeats are frequently found in eukaryotic genomes. High‐copy DNA repeats can serve as building blocks of complex DNA structures, but the in vitro synthesis of DNA repeats has been challenging due to complicated procedures and the high cost. Here, a new, simple method is developed using the strategy of blocking polymerase chain reaction for highly efficient DNA repeat expansion (BPRE). With BPRE, dsDNA fragments composed of more than 40 copies of the repeat sequence can be quickly produced, while the cost is reduced by at least 90%. As a typical application, reannealing of the dsDNA repeats generates elastic hydrogels, which shows a high capacity for doxycycline absorption and prolonged release. Abstract : High‐copy DNA repeats are building blocks of complex DNA superstructures with special features. Synthesis of the DNA repeats has been complicated and expensive. Based on the simple strategy of blocking polymerase chain reaction (PCR), the newly invented "blocking PCR for DNA repeat expansion" method now makes mass productions of the high‐copy DNA repeats fast, cheap, and clean.