Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation. (24th October 2020)

• Record Type: Journal Article
• Authors: Zhang, Ning; Chang, Yong‐Gang; Tseng, Roger; Ovchinnikov, Sergey; Schwarz, Rakefet; LiWang, Andy
• Is Part Of: Protein science. Volume 29:Number 11(2020)
• Page Start: 2274
• Page End: 2280
• Publication Date: 2020-10-24
• Subjects: biofilm -- cyanobacteria -- NMR spectroscopy -- protein structure -- S. elongatus PCC 7942; Proteins -- Periodicals; 572.6
• DOI: 10.1002/pro.3952
• Languages: English
• ISSNs: 0961-8368

Abstract

Abstract: Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four‐stranded β‐sheet that is flanked by four α‐helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs. Abstract : PDB Code(s): 6UF2 ;