Determination and pharmacokinetic analysis of ticarcillin disodium–clavulanate potassium for injection in rat plasma by UPLC-ESI-MS/MS. Issue 12 (December 2020)

• Record Type: Journal Article
• Authors: Wang, Moli; Gao, Yanxia; Liu, Xueli; Zhang, Jing; Wang, Qiang; Chang, Junshan; Zhang, Lantong
• Is Part Of: Journal of international medical research. Volume 48:Issue 12(2020)
• Publication Date: 2020-12
• Subjects: Clavulanate potassium -- in vivo -- pharmacokinetics -- ticarcillin -- ultra-high-performance liquid chromatography -- mass spectrometry -- rat; Medicine -- Periodicals; Pharmacology -- Periodicals; 610.5
• DOI: 10.1177/0300060520967822
• Languages: English
• ISSNs: 0300-0605

Abstract

Objective: To establish a specific and rapid ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UPLC-ESI-MS/MS) method for measuring ticarcillin and clavulanate levels in rat plasma. Methods: A Waters ACQUITY BEH C18 column (50 mm × 2.1 mm, 1.7 μm) and SCIEX QTRAP® LC-MS/MS System were used. Analyses were conducted to optimize the chromatographic and MS conditions, and the pharmacokinetic parameters of ticarcillin and clavulanate were assessed. Results: Linear relationships were observed in the ranges of 10 to 10, 000 ng/mL for ticarcillin R (r 2 = 0.9967) 30 to 10, 000 ng/mL for ticarcillin S (r 2 = 0.9961), and 30 to 10, 000 ng/mL for clavulanate (r 2 = 0.9981). The average extraction recoveries of all compounds ranged from 86.9% to 96.4%. The pharmacokinetic parameters of the ticarcillin R and S isomers in rats were distinctive. The ticarcillin R and S isomers and clavulanate were rapidly absorbed in vivo . Ticarcillin S and clavulanate had similar elimination rates, whereas that of ticarcillin R was slower. Conclusion: A UPLC-ESI-MS/MS method was developed and validated for the determination of ticarcillin and clavulanate in rat plasma.