Fluorescently labeled bacteria provide insight on post-mortem microbial transmigration. (July 2016)

• Record Type: Journal Article
• Authors: Burcham, Z.M.; Hood, J.A.; Pechal, J.L.; Krausz, K.L.; Bose, J.L.; Schmidt, C.J.; Benbow, M.E.; Jordan, H.R.
• Is Part Of: Forensic science international. Volume 264(2016)
• Page Start: 63
• Page End: 69
• Publication Date: 2016-07
• Subjects: Forensics -- Staphylococcus aureus -- Clostridium perfringens -- Necrobiome -- Decomposition; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2016.03.019
• Languages: English
• ISSNs: 0379-0738

Abstract

Highlights: Staphylococcus aureus transmigration was detected throughout murine decomposition. S. aureus abundance peaked 5–7 days post decomposition. Naturally sterile organs showed undulating S. aureus positivity during decomposition. S. aureus was widely distributed in organs in non-surface sterilized mice. Post-mortem microbial transmigration may be useful in providing PMI estimates. Abstract: Microbially mediated mechanisms of human decomposition begin immediately after death, and are a driving force for the conversion of a once living organism to a resource of energy and nutrients. Little is known about post-mortem microbiology in cadavers, particularly the community structure of microflora residing within the cadaver and the dynamics of these communities during decomposition. Recent work suggests these bacterial communities undergo taxa turnover and shifts in community composition throughout the post-mortem interval. In this paper we describe how the microbiome of a living host changes and transmigrates within the body after death thus linking the microbiome of a living individual to post-mortem microbiome changes. These differences in the human post-mortem from the ante-mortem microbiome have demonstrated promise as evidence in death investigations. We investigated the post-mortem structure and function dynamics of Staphylococcus aureus and Clostridium perfringens after intranasal inoculation in the animal model Mus musculus L. (mouse) to identify how transmigration of bacterial species can potentially aid in post-mortem interval estimations. S. aureus was tracked using in vivo and in vitro imaging to determine colonization routes associated with different physiological events of host decomposition, while C. perfringens was tracked using culture-based techniques. Samples were collected at discrete time intervals associated with various physiological events and host decomposition beginning at 1 h and ending at 60 days post-mortem. Results suggest that S. aureus reaches its highest concentration at 5–7 days post-mortem then begins to rapidly decrease and is undetectable by culture on day 30. The ability to track these organisms as they move in to once considered sterile space may be useful for sampling during autopsy to aid in determining post-mortem interval range estimations, cause of death, and origins associated with the geographic location of human remains during death investigations.