Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry. Issue 21 (26th August 2020)

• Record Type: Journal Article
• Authors: Chen, Liang; Cabot, Joan M.; Sanz Rodriguez, Estrella; Ghiasvand, Alireza; Innis, Peter C.; Paull, Brett
• Is Part Of: Analyst. Volume 145:Issue 21(2020)
• Page Start: 6928
• Page End: 6936
• Publication Date: 2020-08-26
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an01344g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Efficient 'on-thread' isoelectric focusing of proteins, with direct on-thread detection using desorption electrospray ionisation mass spectrometry. Abstract : The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct "on-thread" ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.