Withania somnifera dietary supplementation improves lipid profile, intestinal histomorphology in healthy Nile tilapia (Oreochromis niloticus), and modulates cytokines response to Streptococcus infection. Issue 106 (November 2020)

• Record Type: Journal Article
• Authors: Zahran, Eman; El Sebaei, Mahmoud G.; Awadin, Walaa; Elbahnaswy, Samia; Risha, Engy; Elseady, Youssef
• Is Part Of: Fish & shellfish immunology. Issue 106(2020)
• Page Start: 133
• Page End: 141
• Publication Date: 2020-11
• Subjects: Fish -- Herbal plant -- Lipid metabolism -- Gut morphology -- Gene expression -- Bacterial challenge; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.07.056
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Despite Withania somnifera ( WS ), stimulating effects have been investigated on many animal species, its role on lipid profile and intestinal histomorphology in healthy animals, and its modulating role on pro-inflammatory cytokines following infection in fish are yet scarce. In this context, lipid profile, liver, and intestinal histomorphology were measured in Nile tilapia fed with a basal diet or diets containing 2.5 and 5% of supplementary WS for 60 days. Besides, cytokines response was measured at 1, 3, 7, and 14 days following Streptococcus iniae (S. iniae) infection after the feeding trial. All lipid profile parameters were nominally lowered, excluding high-density lipoprotein (HDL) that exhibited a significant increase in WS 5% group compared to other groups. Improved gut health integrity was observed, especially in WS 5% group in terms of increased goblet cell numbers, villous height, the width of lamina propria in all parts of the intestine, and a decrease in the diameter of the intestinal lumen of the distal intestine only. A significant down-regulation in the mRNA transcript level of cytokine genes (interleukin 1β/IL-1β, tumor necrosis factor α/TNFα, and interleukin 6/IL-6) was demonstrated in the kidney and spleen of WS -supplemented groups following S. iniae infection compared with the control infected (positive control/PC) group. Our findings give new insights for the potential roles of WS dietary inclusion not only on lipid profile and intestinal health integrity improvement in healthy fish under normal rearing but also as a prophylactic against the infection. Thus, WS can be incorporated as a promising nutraceutical in aquaculture. Highlights: Withania somnifera ( WS) can be incorporated in fish diets as safe functional feeds. WS did not have any negative impacts on lipid profile and tissue histology of Nile tilapia. WS enhanced high density lipoprotein in Nile tilapia, particularly at dose of 5%. WS supplementation improves gut health in Nile tilapia. WS protect against the inflammation induced during streptococcal infection in tilapia.