Characterization and immune function of decapentaplegic (Dpp) gene from the oriental river prawn, Macrobrachium nipponense. Issue 106 (November 2020)

• Record Type: Journal Article
• Authors: Xu, Liaoyi; Wu, Yue; Zhou, Xiefei; Wang, Peichen; Xu, Haisheng
• Is Part Of: Fish & shellfish immunology. Issue 106(2020)
• Page Start: 804
• Page End: 813
• Publication Date: 2020-11
• Subjects: Macrobrachium nipponense -- Decapentaplegic -- TGF-β superfamily -- Immune response -- NF-κB signaling; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.08.044
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The Decapentaplegic (Dpp) gene, which belongs to the TGF‐β superfamily, is involved in multiple developmental processes in eukaryotic species. In this study, we firstly identified and characterized Dpp from Macrobrachium nipponense . Its full-length open reading frame (ORF) cDNA was 1332 bp, encoding 443 amino acids. The putative Mn Dpp protein contained a signal peptide, a TGF-β propeptide region and a TGF-β domain. Its TGF-β domain was highly conserved from vertebrates to invertebrates, and exhibited highly similarity to Dpp derived from Bombyx mori . qRT-PCR analysis suggested that MnDpp expressed in all tested tissues and responded to both bacterial and virus pathogens, indicating Mn Dpp was involved in the innate immune response of M. nipponense . Knockdown of Mn Dpp in vivo significantly increased bacteria growth and markedly decreased the expressions of NF-κB signaling genes including dorsal, relish, TAK1, TAB1, Ikkβ and Ikkε as well as antimicrobial peptides (AMPs) including ALF2, ALF3, ALF4, ALF5, Cru1 and Cru2. Moreover, in vitro overexpression of Mn Dpp protein in HEK293T cells further demonstrated that it exerted antibacterial immune response by activation of NF-κB signaling cascade. In summary, these results indicated that Mn Dpp played an important role in the innate immunity in M. nipponense by modulating NF-κB signaling pathway, which might provide new insights about Dpp in crustaceans and paved the way for a better understanding of the crustacean innate immune system. Highlights: Dpp was firstly identified and characterized in crustacean species. Mn Dpp is up-regulated after bacterial and virus challenge in prawns. Mn Dpp could clear A. hydrophilain in M. nipponense. Mn Dpp exert antibacterial function by modulation of NF-κB signaling cascade and AMPs expression.