Bio-electrochemical COD removal for energy-efficient, maximum and robust nitrogen recovery from urine through membrane aerated nitrification. (15th October 2020)

• Record Type: Journal Article
• Authors: De Paepe, Jolien; De Paepe, Kim; Gòdia, Francesc; Rabaey, Korneel; Vlaeminck, Siegfried E.; Clauwaert, Peter
• Is Part Of: Water research. Volume 185(2020)
• Publication Date: 2020-10-15
• Subjects: Resource recovery -- Regenerative life support system -- Nitrogen recovery -- Yellow water -- Source separation -- Membrane biofilm reactor; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2020.116223
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: 85% COD & 100% urea removal from fresh urine with a microbial electrolysis cell. Full urine nitrification in membrane-aerated reactor with DO<0.1 mg O2 L −1 . Bio-anodic pre-treatment avoids N loss in membrane-aerated nitrification. The two reactors contained very distinct and diverse microbial communities. MEC and MABR treatment enables highly resource-efficient urine stabilization. Abstract: Resource recovery from source-separated urine can shorten nutrient cycles on Earth and is essential in regenerative life support systems for deep-space exploration. In this study, a robust two-stage, energy-efficient, gravity-independent urine treatment system was developed to transform fresh real human urine into a stable nutrient solution. In the first stage, up to 85% of the COD was removed in a microbial electrolysis cell (MEC), converting part of the energy in organic compounds (27–46%) into hydrogen gas and enabling full nitrogen recovery by preventing nitrogen losses through denitrification in the second stage. Besides COD removal, all urea was hydrolysed in the MEC, resulting in a stream rich in ammoniacal nitrogen and alkalinity, and low in COD. This stream was fed into a membrane-aerated biofilm reactor (MABR) in order to convert the volatile and toxic ammoniacal nitrogen to non-volatile nitrate by nitrification. Bio-electrochemical pre-treatment allowed to recover all nitrogen as nitrate in the MABR at a bulk-phase dissolved oxygen level below 0.1 mg O2 L −1 . In contrast, feeding the MABR directly with raw urine (omitting the first stage), at the same nitrogen loading rate, resulted in nitrogen loss (18%) due to denitrification. The MEC and MABR were characterised by very distinct and diverse microbial communities. While (strictly) anaerobic genera, such as Geobacter (electroactive bacteria), Thiopseudomonas, a Lentimicrobiaceae member, Alcaligenes and Proteiniphilum prevailed in the MEC, the MABR was dominated by aerobic genera, including Nitrosomonas (a known ammonium oxidiser), Moheibacter and Gordonia . The two-stage approach yielded a stable nitrate-rich, COD-low nutrient solution, suitable for plant and microalgae cultivation. Graphical abstract: Image, graphical abstract