An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli. Issue 20 (15th October 2020)

• Record Type: Journal Article
• Authors: Zambaldo, Claudio; Koh, Minseob; Nasertorabi, Fariborz; Han, Gye Won; Chatterjee, Abhishek; Stevens, Raymond C.; Schultz, Peter G.
• Is Part Of: Bioorganic & medicinal chemistry. Volume 28:Issue 20(2020)
• Publication Date: 2020-10-15
• Subjects: Seryl-tRNA synthetase -- E. coli orthogonality -- Genetic code expansion -- X-ray crystallography -- Non-canonical amino acids; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2020.115662
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNA Ser )/ Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli . Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).