Characterization and antimicrobial evaluation of a new Spgly-AMP, glycine-rich antimicrobial peptide from the mud crab Scylla paramamosain. Issue 106 (November 2020)

• Record Type: Journal Article
• Authors: Xie, Yichao; Wan, Haifu; Zeng, Xianyuan; Zhang, Ziping; Wang, Yilei
• Is Part Of: Fish & shellfish immunology. Issue 106(2020)
• Page Start: 384
• Page End: 392
• Publication Date: 2020-11
• Subjects: gly-AMP -- Vibrio parahaemolyticus -- PGN -- Poly I:C -- Expression pattern -- Antimicrobial activity -- Scylla paramamosain; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.08.009
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Antimicrobial peptide (AMP) is a crucial component of the innate immune system in crustaceans. In mud crab, Scylla paramamosain, a commercially important species, a glycine-rich antimicrobial peptide ( Sp gly-AMP) gene was newly identified and putatively encoded a 26aa signal peptide and 37aa mature peptide. To understand the function of Sp gly-AMP, the expression profile of Spgly-amp gene was characterized, which showed Spgly-amp was expressed widely in most tissues of adult crabs with the highest expression level in hemocytes. After Vibrio parahaemolyticus, PGN, or Poly I:C stimulations, the expression level of Spgly - amp was significantly up-regulated in the hemocytes. In antimicrobial assays, chemically synthesized Sp gly-AMP peptides exhibited strong antibacterial activities against both Gram-positive and Gram-negative bacteria and high thermal stability after high-temperature heating. These findings in the present study verified the importance of the Sp gly-AMP in defense of pathogenic bacteria infection in the mud crab and provided a promising candidate of antimicrobial agents in the crab aquaculture. Highlights: A glycine-rich antimicrobial peptide ( Sp gly-AMP) gene was newly identified from mud crab. The orthologs of gly-AMP in several crabs are highly conserved. After bacterial stimulations, the Spgly - amp mRNA was significantly induced in the hemocytes. Sp gly-AMP peptides exhibit strong antibacterial activities and high thermal stability.