High‐Throughput Imaging: Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle‐Resolved Subvoxel SPIM (Adv. Sci. 3/2020). Issue 3 (5th February 2020)

• Record Type: Journal Article
• Authors: Nie, Jun; Liu, Sa; Yu, Tingting; Li, Yusha; Ping, Junyu; Wan, Peng; Zhao, Fang; Huang, Yujie; Mei, Wei; Zeng, Shaoqun; Zhu, Dan; Fei, Peng
• Is Part Of: Advanced science. Volume 7:Issue 3(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-05
• Subjects: brain imaging -- computational imaging -- light‐sheet fluorescence microscopy -- neuroscience -- super resolution; Science -- Periodicals; 505
• DOI: 10.1002/advs.202070015
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract : In article number 1901891, Dan Zhu, Peng Fei, and co‐workers develop an imaging technique, multiangle‐resolved subvoxel selective plane illumination microscopy (termed Mars‐SPIM), that can readily obtain the 3D digital atlas of a cleared whole mouse brain with an isotropic single‐cell resolution, in a short acquisition time of several minutes. It provides an efficient way to implement system‐level cellular analysis, such as the mapping of different neuron populations and tracing of long‐distance neural projections over the entire brain.