Long non-coding RNAs mortal obligate RNA transcript regulates the proliferation of human periodontal ligament stem cells and affects the recurrence of periodontitis. (September 2019)

• Record Type: Journal Article
• Authors: Wang, Yanhua; Sun, Yingying; Zheng, Peng; Cai, Chunyu; Jiang, Yu; Zhang, Huiyan; Li, Zuntai; Cai, Qing
• Is Part Of: Archives of oral biology. Volume 105(2019)
• Page Start: 1
• Page End: 4
• Publication Date: 2019-09
• Subjects: Periodontitis -- Periodontal ligament stem cells -- lncRNA MORT -- Proliferation; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2019.04.013
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: lncRNA MORT overexpression inhibits human periodontal ligament stem cells. Level of lncRNA MORT affects the recurrence of periodontitis. LncRNA MORT is a therapeutic target for human periodontal ligament stem cells. Abstract: Objective: The aim of this study is to investigate the role of long non-coding RNAs (lncRNA), mortal obligate RNA transcript (MORT), in human periodontal ligament stem cells. Design: Periodontal ligament tissues were collected from 48 periodontitis patients underwent tooth extraction and 38 people in similar age and gender distributions who experienced orthodontic treatment. After treatment, periodontitis patients were followed up for 2 years. MORT in periodontal ligament stem cells (PDLSCs) was detected by qRT-PCR. Proliferation of PDLSCs was detected by CCK-8 assay. Results: The proliferation rate of PDLSCs isolated from periodontitis-affected teeth was significantly higher than PDLSCs from healthy teeth. Overexpression of MORT inhibited the proliferation of both types of periodontal ligament stem cells. After treatment, periodontitis patients were followed up for 2 years and patients with high level of MORT expression showed relatively lower recurrence rate comparing to low expression group. Conclusion: MORT is involved in the proliferation of human PDLSCs and affects the recurrence of periodontitis.