Responses to diagnostic tests for bovine tuberculosis in dairy and non-dairy cattle naturally exposed to Mycobacterium bovis in Great Britain. (October 2016)

• Record Type: Journal Article
• Authors: Downs, S.H.; Broughan, J.M.; Goodchild, A.V.; Upton, P.A.; Durr, P.A.
• Is Part Of: Veterinary journal. Volume 216(2016)
• Page Start: 8
• Page End: 17
• Publication Date: 2016-10
• Subjects: Bovine tuberculosis -- Dairy cattle -- Diagnosis -- Single intradermal comparative cervical tuberculin test; Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2016.06.010
• Languages: English
• ISSNs: 1090-0233

Abstract

Highlights: Dairy reactor cattle in Great Britain are less likely to have Mycobacterium bovis infection confirmed than non-dairy cattle. Tuberculin-based tests are less predictive of post-mortem confirmed M. bovis infection in dairy than in non-dairy cattle. Defined antigen interferon-γ blood tests may be more specific for M. bovis infection than tuberculin-based tests. Abstract: Field surveillance of British cattle using the single intradermal comparative cervical tuberculin (SICCT) test shows a higher incidence rate of bovine tuberculosis (bTB) in dairy compared to beef herds, but a lower probability of post-mortem examination confirmed (PMC) Mycobacterium bovis infection in dairy herds. A cross-sectional study was conducted to compare animal level differences in bTB detection between dairy and non-dairy cattle in Great Britain. During the period from 2002 to 2005, 200 (41% dairy) reactors in the SICCT test (standard interpretation) were randomly selected, and 200 in-contact cattle (43% dairy) were purposively selected from bTB-infected herds. Interferon (IFN)-γ responses in blood to bovine and avian purified protein derivative (PPD), and early secretory antigen target 6 kDa and culture filtrate protein 10 (ESAT-6/CFP10), were measured. The post-mortem examination included gross pathological examination, mycobacterial culture and histopathology. The proportions of cattle positive to ESAT6/CFP10 were 26% (95% confidence interval, CI, 15–39%) in dairy reactors and 62% (95% CI 51–72%) in non-dairy reactors ( P < 0.001). PMC risk was 34% (95% CI 24–45%) in dairy reactors and 69% (95% CI 60–78%) in non-dairy reactors ( P < 0.001). The odds ratio for PMC risk in dairy reactors compared to non-dairy reactors, after controlling for bTB prevalence, herd size and SICCT test response, was 0.27 (95% CI 0.14–0.53; P < 0.001). In surveillance data, adjusted animal level PMC risks were lower for dairy reactors than for beef reactors aged >2 years ( P < 0.001).