Expression of microRNAs miR-21 and miR-181c in cerebrospinal fluid and serum in canine meningoencephalomyelitis of unknown origin. (October 2016)

• Record Type: Journal Article
• Authors: Gaitero, L.; Russell, S.J.; Monteith, G.; LaMarre, J.
• Is Part Of: Veterinary journal. Volume 216(2016)
• Page Start: 122
• Page End: 124
• Publication Date: 2016-10
• Subjects: Canine -- Meningoencephalomyelitis of unknown origin -- Cerebrospinal fluid -- MicroRNAs; Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2016.07.014
• Languages: English
• ISSNs: 1090-0233

Abstract

Highlights: MicroRNAs were investigated as potential biomarkers in canine meningoencephalitis of unknown origin (MUO). MicroRNA-21 and microRNA-181c were detected in cerebrospinal fluid (CSF) and serum of dogs with MUO. Expression of both microRNAs was higher in CSF of dogs with MUO than dogs with non-inflammatory neurological diseases. Expression of microRNAs was correlated with cellularity of CSF. Abstract: The potential of microRNAs (miRNAs) as biomarkers for canine meningoencephalomyelitis of unknown origin (MUO) was investigated by using quantitative real-time (qRT)-PCR to determine the expression of microRNA-21 (miR-21) and microRNA-181c (miR-181c) in the cerebrospinal fluid (CSF) of dogs. Dogs with MUO ( n = 10) had higher levels of expression of miR-21 and miR-181c in the CSF than dogs with non-inflammatory neurological diseases ( n = 8). There was a positive correlation between CSF cellularity and expression of miRNAs in the CSF, particularly for miR-21 in the MUO group.