The functional diversity of Drosophila Ino80 in development. (November 2015) - Ghasemi, Mohsen
Abstract: Ino80 is well known as a chromatin remodeling protein with the catalytic function of DNA dependent ATPase and is highly conserved across phyla. Ino80 in human and Drosophila is known to form the Ino80 complex in association with the DNA binding protein Ying–Yang 1 (YY1)/Pleiohomeotic (Pho) the Drosophila homologue. We have earlier reported that Ino80 sub-family of proteins has two functional domains, namely, the DNA dependent ATPase and the DNA binding domain. In the background of the essential role of dIno80 in development, we provide evidence of Pho independent function of dIno80 in development and analyze the dual role of dIno80 in activation as well as repression in the context of the homeotic gene Scr (sex combs reduced) in imaginal discs. This differential effect of dIno80 in different imaginal discs suggests the contextual function of dIno80 as an Enhancer of Trithorax and Polycomb (ETP). We speculate on the role of dIno80 as a chromatin remodeler on one hand and a potential recruiter of epigenetic regulatory complexes on the other. Graphical abstract: Highlights: Chromatin remodeling protein, dIno80, is a developmental gene regulator. It regulates homeotic gene expression. dIno80 can act as a positive and negative regulator of the expression of Scr . dIno80 plays a role in development distinct and independent of pleiohomeotic gene. dIno80 functions as an ETP gene and interacts with both PcG and TrxG genes.