Surface characteristics of a modified acidulated phosphate fluoride gel with nano‐hydroxyapatite coating applied on bovine enamel subjected to an erosive environment. Issue 12 (8th October 2018)

• Record Type: Journal Article
• Authors: Soares, Luís Eduardo Silva; da Silva Magalhães, Juarez; Marciano, Fernanda Roberta; Lobo, Anderson Oliveira
• Is Part Of: Microscopy research and technique. Volume 81:Issue 12(2018)
• Page Start: 1456
• Page End: 1466
• Publication Date: 2018-10-08
• Subjects: enamel -- energy dispersive X‐ray fluorescence spectrometry -- fluoride -- hydroxyapatite -- scanning electron microscopy; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23146
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract : This study evaluated the antierosive effect applying a modified acidulated phosphate fluoride (APF) gel containing nano‐hydroxyapatite (nHAp) on the enamel surface before erosion. After polishing, the exposed flat enamel surfaces ( n = 7/group) from bovine incisors were treated with artificial saliva (S ‐ negative control), orange juice (ERO), APF gel (positive control) and APF_nHAp gel. All samples were subjected to six cycles of demineralization (orange juice, pH ~ 3.5, 10 min) followed by remineralization (saliva, 37°C, 1 hr). The enamel surface morphology, topography, and inorganic composition were analyzed using scanning electron microscopy, roughness testing, and micro energy‐dispersive X‐ray fluorescence spectrometry, respectively. The mean (standard deviation) roughness values (Ra, μm) were S, 0.13 (0.05); ERO, 0.25 (0.07); APF, 0.22 (0.08); and APF_nHAp, 0.17 (0.04). Ra values were significantly higher after ERO ( p < .01) and APF ( p < .05) treatments than after S. The enamel surface morphology was altered by the treatments, except for the S specimens. The mineral content of the enamel showed a clear trend with Ca and P reduction in the order of APF < S < APF_nHAp < ERO and APF < S < ERO < APF_nHAp, respectively. We can conclude that APF gel increased mineral concentration on the enamel. Moreover, the APF_nHAp material modified the composition and morphology of the enamel surface. Abstract : APF gel resulted in increased mineral concentration on the enamel. APF_nHAp modified the composition and morphology of the enamel surface. Combined SEM‐μ‐EDXRF‐roughness analyses allow monitoring of enamel coatings.