A PCR-free technology to detect and quantify microRNAs directly from human plasma. Issue 23 (9th November 2018)

• Record Type: Journal Article
• Authors: Marín-Romero, Antonio; Robles-Remacho, Agustín; Tabraue-Chávez, Mavys; López-Longarela, Bárbara; Sánchez-Martín, Rosario M.; Guardia-Monteagudo, Juan J.; Fara, Mario A.; López-Delgado, Francisco J.; Pernagallo, Salvatore; Díaz-Mochón, Juan J.
• Is Part Of: Analyst. Volume 143:Issue 23(2018)
• Page Start: 5676
• Page End: 5682
• Publication Date: 2018-11-09
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01397g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel and unique method for a PCR/label-free detection and absolute quantification of miR-451a in blood samples. Abstract : A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.