Evaluation of Natural and Synthetic Phosphate Donors for the Improved Enzymatic Synthesis of Phosphate Monoesters. Issue 12 (2nd May 2018)

• Record Type: Journal Article
• Authors: Tasnádi, Gábor; Jud, Wolfgang; Hall, Mélanie; Baldenius, Kai; Ditrich, Klaus; Faber, Kurt
• Is Part Of: Advanced synthesis & catalysis. Volume 360:Issue 12(2018)
• Page Start: 2394
• Page End: 2401
• Publication Date: 2018-05-02
• Subjects: Enzymatic phosphorylation -- phosphatase -- phosphate donor -- acetyl phosphate; Catalysis -- Periodicals; Organic compounds -- Synthesis -- Periodicals; Chemistry -- Periodicals; Chemistry, Technical -- Periodicals; Chemistry -- Periodicals; Catalysis -- Periodicals; Technology, Pharmaceutical -- Periodicals; 547.2
• DOI: 10.1002/adsc.201800306
• Languages: English
• ISSNs: 1615-4150

Abstract

Abstract: Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by‐product are the main obstacles associated with the use of pyrophosphate in the phosphatase‐catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both limitations, we screened a broad range of natural and synthetic organic phosphate donors with several enzymes on a broad variety of hydroxyl‐compounds. Among them, acetyl phosphate delivered stable product levels and high phospho‐transfer efficiency at the lower functional pH‐limit, which translated into excellent productivity. The protocol is generally applicable to acid phosphatases and compatible with a range of diverse substrates. Preparative‐scale transformations using acetyl phosphate synthesized from cheap starting materials yielded multiple grams of various sugar phosphates with up to 433 g L −1 h −1 space‐time yield and 75% reduction of barium phosphate waste. Abstract :