Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation. (September 2015)

• Record Type: Journal Article
• Authors: Cerrone, Federico; Davis, Reeta; Kenny, Shane T.; Woods, Trevor; O'Donovan, Anthonia; Gupta, Vijai Kumar; Tuohy, Maria; Babu, Ramesh P.; O'Kiely, Padraig; O'Connor, Kevin
• Is Part Of: Bioresource technology. Volume 191(2015)
• Page Start: 45
• Page End: 52
• Publication Date: 2015-09
• Subjects: Biorefining -- Ensiled grass press juice -- Polyhydroxyalkanoates -- Mannitol -- Pulse feed; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.04.128
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: First report on the use of a mannitol rich renewable carbon source for PHA production. Achieved PHB and mcl-PHA production using EGPJ as sole carbon source. EGPJ is a suitable substrate to achieve high cell density and high PHB productivity. PHB productivity on EGPJ was comparable to that on commercial sugars. Abstract: This study demonstrates the use of a mannitol rich ensiled grass press juice (EGPJ) as a renewable carbon substrate for polyhydroxyalkanoates (PHA) production in shaking flask experiments and fed-batch stirred tank reactor cultivations. Fed-batch cultivations of Burkholderia sacchari IPT101 using EGPJ as sole carbon source produced 44.5 g/L CDW containing 33% polyhydroxybutyrate (PHB) in 36 h, while Pseudomonas chlororaphis IMD555 produced a CDW of 37 g/L containing 10% of medium chain length polyhydroxyalkanoates (mcl-PHA) in 34 h. PHB and mcl-PHA extracted from B. sacchari IPT101 and P. chlororaphis IMD555, grown on EGPJ, had a molecular weight of 548 kg/mol and 115.4 kg/mol, respectively. While mcl-PHA can be produced from EGPJ, PHB production is more interesting as there is a 4-fold higher volumetric productivity compared to mcl-PHA.