SiRNA release from gold nanoparticles by nanosecond pulsed laser irradiation and analysis of the involved temperature increase. Issue 9 (24th May 2018)

• Record Type: Journal Article
• Authors: Rudnitzki, Florian; Feineis, Susanne; Rahmanzadeh, Ramtin; Endl, Elmar; Lutz, Johanna; Groll, Jürgen; Hüttmann, Gereon
• Is Part Of: Journal of biophotonics. Volume 11:Issue 9(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-24
• Subjects: cavitation -- cell manipulation -- controlled release -- gold nanoparticle bio‐conjugates -- laser nanoeffects; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201700329
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Nanosecond pulsed laser irradiation can trigger a release of nucleic acids from gold nanoparticles, but the involved nanoeffects are not fully understood yet. Here we investigate the release of coumarin labeled siRNA from 15 to 30 nm gold particles after nanosecond pulsed laser irradiation. Temperatures in the particle and near the surface were calculated for the different radiant exposures. Upon irradiation with laser pulses of 4 nanosecond duration release started for both particle sizes at a calculated temperature increase of approximately 500 K. Maximum coumarin release was observed for 15 nm particles after irradiation with radiant exposure of 80 mJ cm −2 and with 32 mJ cm −2 for 30 nm particles. This corresponds to a temperature increase of 815 and 900 K, respectively. Our results show that the molecular release by nanosecond pulsed irradiation is based on a different mechanism compared to continuous or femtosecond irradiation. Local temperatures are considerably higher and it is expected that bubble formation plays a crucial role in release and damage to cellular structures. Abstract : Controlled release of DNA or RNA at specific sites is of fundamental interest for selective therapies or basic studies in cell biology. Light‐controlled release allows high spatial and temporal definition of biological effects affected by DNA or RNA. Short pulsed laser irradiation can trigger the release of nucleic acids from gold nanoparticles, which have shown to precisely target cellular structures. Our investigation shows that the molecular release by nanosecond pulsed irradiation is based on a different mechanism compared to continuous or femtosecond irradiation. Local temperatures are considerably higher and bubble formation plays a crucial role in siRNA release and unwanted damage to the siRNA as well as the surrounding cellular structures.