Acteoside protects against 6-OHDA-induced dopaminergic neuron damage via Nrf2-ARE signaling pathway. (September 2018)

• Record Type: Journal Article
• Authors: Li, Maiquan; Zhou, Fei; Xu, Tao; Song, Huaxin; Lu, Baiyi
• Is Part Of: Food and chemical toxicology. Volume 119(2018)
• Page Start: 6
• Page End: 13
• Publication Date: 2018-09
• Subjects: Acteoside -- Nrf2 -- Parkinson's disease -- The blood-brain-barrier -- Zebrafish; 6-OHDA 6-hydroxydopamine -- ARE antioxidant response elements -- BBB the blood-brain-barrier -- CAT catalase -- DA dopaminergic -- dpf day post fertilization -- GLCM glutathione cysteine ligase modulatory subunit -- GCLC glutathione cysteine ligase regulatory subunit -- GSH-Px glutathione peroxidase -- HO-1 heme oxygenase-1 -- Keap1 Kelch-like ECH-associated protein 1 -- MDA lipid peroxidation -- Nrf2 nuclear factor erythroid 2-related factor 2 -- PD Parkinson's disease -- PDA photodiode array -- SOD superoxide dismutase -- TH tyrosine hydroxylase; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.06.018
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Acteoside has been reported to have antioxidant and neuroprotective effect, which is a promising therapeutic way in prevention and treatment of Parkinson's disease. The present study was aimed to understand the neuroprotective effect of acteoside and to elucidate its underlying mechanism. 6-hydroxydopamine (6-OHDA)-induced neural damage in zebrafish model was used to study the protective effect of acteoside on Parkinson's disease (PD). Locomotion behavioral test showed that acteoside could prevent 6-OHDA-stimulated movement disorders. Anti-tyrosine hydroxylase (TH) whole-mount immunostaining analysis showed that acteoside could prevent 6-OHDA-induced dopaminergic neuron death. In addition, pretreatment with acteoside could upregulate antioxidative enzymes by activating the Nrf2/ARE signaling pathway in zebrafish. Meanwhile, acteoside was found to be distributed in the brain after intraperitoneal injection into the adult zebrafish, indicating that this compound could penetrate the blood-brain-barrier (BBB). This study demonstrated that acteoside could penetrate BBB and have potential therapeutic value for PD by activating the Nrf2/ARE signaling pathway and attenuating the oxidative stress. Graphical abstract: Highlights: Acteoside showed neuroprotective effect of on 6-OHDA-induced zebrafish PD model. Acteoside activated the Nrf2/ARE signaling pathway in zebrafish PD model. Acteoside could penetrate the blood-brain-barrier in zebrafish.