Separation of Nucleobases Using High‐performance Liquid Chromatography Coupled with Voltammetric Scanning. Issue 8 (26th April 2018)

• Record Type: Journal Article
• Authors: Zitka, Ondrej; Kudr, Jiri; Labuda, Jan; Xhaxhiu, Kledi; Adam, Vojtech
• Is Part Of: Electroanalysis. Volume 30:Issue 8(2018)
• Page Start: 1857
• Page End: 1861
• Publication Date: 2018-04-26
• Subjects: Glassy carbon electrode -- On-line voltammetry scan -- Nucleic acid -- Nucleobases -- Reverse phase; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201800175
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: In this paper, a method based on chromatographic separation of analytes and their quantification using on‐line cyclic voltammetry is reported. The method based on high performance liquid chromatography on reverse phase column was optimized using free nucleobases‐guanine, adenine, thymine, and cytosine. The optimal separation of nucleobases was detected when using 0.05 M borate buffer (pH 9.0) as the mobile phase, at isocratic flow rate 1 mL min −1, and at separation column temperature of 30 °C. The accurate timing of the cyclic voltammetry enabled us to quantify the concentrations of individual nucleobases by oxidation on glassy carbon electrode. Abstract :