Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues. (June 2017)

• Record Type: Journal Article
• Authors: Tsapekos, P.; Kougias, P.G.; Vasileiou, S.A.; Treu, L.; Campanaro, S.; Lyberatos, G.; Angelidaki, I.
• Is Part Of: Bioresource technology. Volume 234(2017)
• Page Start: 350
• Page End: 359
• Publication Date: 2017-06
• Subjects: Bioaugmentation -- Clostridium thermocellum -- Melioribacter roseus -- Methane yield -- High throughput sequencing; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.03.043
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Bioaugmentation with Clostridium thermocellum and Melioribacter roseus was examined. C. thermocellum enhanced the anaerobic biodegradability of wheat straw up to 34%. M. roseus had limited efficiency and boosted the methane yield by only 11%. C. thermocellum can be used to extract the residual methane of the reactor. Bioaugmentation did not alter markedly the indigenous microbial communities. Abstract: Bioaugmentation with hydrolytic microbes was applied to improve the methane yield of bioreactors fed with agricultural wastes. The efficiency of Clostridium thermocellum and Melioribacter roseus to degrade lignocellulosic matter was evaluated in batch and continuously stirred tank reactors (CSTRs). Results from batch assays showed that C. thermocellum enhanced the methane yield by 34%. A similar increase was recorded in CSTR during the bioaugmentation period; however, at steady-state the effect was noticeably lower (7.5%). In contrast, the bioaugmentation with M. roseus did not promote markedly the anaerobic biodegradability, as the methane yield was increased up to 10% in batch and no effect was shown in CSTR. High-throughput 16S rRNA amplicon sequencing was used to assess the effect of bioaugmentation strategies on bacterial and archaeal populations. The microbial analysis revealed that both strains were not markedly resided into biogas microbiome. Additionally, the applied strategies did not alter significantly the microbial communities.