An integrated microfluidic chip for the detection of bacteria – A proof of concept. Issue 4 (August 2015)

• Record Type: Journal Article
• Authors: Guo, Zhe; Yu, Ting; He, Jiarui; Liu, Fen; Hao, Hualong; Zhao, Yang; Wen, Jiabin; Wang, Qi
• Is Part Of: Molecular and cellular probes. Volume 29:Issue 4(2015)
• Page Start: 223
• Page End: 227
• Publication Date: 2015-08
• Subjects: Microfluidic chip -- Solid phase extraction (SPE) -- Loop-mediated isothermal amplification (LAMP) -- Bacterial detection; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2015.05.005
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: We designed a microfluidic chip as a proof of concept for the detection of bacterial DNA. The chip was fabricated with poly-dimethylsiloxane (PDMS). It included a solid phase extraction (SPE) chamber, two separate channels and multiple loop-mediated isothermal amplification (LAMP) chambers. Three bacterial strains ( Escherichia coli O157 : H7, methicillin-resistant Staphylococcus aureus and methicillin-sensitive S. aureus ) were used to test the feasibility of the device. LAMP products were examined directly using a UV light and verified by agarose gel electrophoresis. Using this chip, we successfully detected E. coli O157 : H7, MSSA and MRSA in less than 2 h. The detection limit for genes rfb E, spa and mec A (specific to E. coli O157 : H7, MSSA and MRSA, respectively) was <10 2 CFU/100 μl. Further work is required to refine this approach and rigorously assess its analytical and diagnostic specificity and sensitivity. Highlights: Microfluidic chip shows its low-cost, disposability, portability and integration. DNA purification, amplification and results judgment were implemented on chip consecutively. Rapid and accurate bacterial detection was implemented on chip.