Clostridium amazonense sp. nov. an obliqately anaerobic bacterium isolated from a remote Amazonian community in Peru. (October 2015)

• Record Type: Journal Article
• Authors: O'Neal, Lindsey; Obregón-Tito, Alexandra J.; Tito, Raul Y.; Ozga, Andrew T.; Polo, Susan I.; Lewis, Cecil M.; Lawson, Paul A.
• Is Part Of: Anaerobe. Volume 35:Part B(2015)
• Page Start: 33
• Page End: 37
• Publication Date: 2015-10
• Subjects: Clostridium amazonense sp. nov. -- Indigenous community -- 16S rRNA phylogeny -- Taxonomy; BD Becton, Dickinson and Company -- CCUG Culture Collection-University of Goteborg -- HMP Human Microbiome Project -- PY peptone-yeast extract; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2015.06.005
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: A strictly anaerobic Gram-stain positive, spore-forming, rod-shaped bacterium designated NE08V T, was isolated from a fecal sample of an individual residing in a remote Amazonian community in Peru. Phylogenetic analysis based on the 16S rRNA gene sequence showed the organism belonged to the genus Clostridium and is most closely related to Clostridium vulturis (97.4% sequence similarity) and was further characterized using biochemical and chemotaxonomic methods. The major cellular fatty acids were anteiso C13:0 and C16:0 with a genomic DNA G + C content of 31.6 mol%. Fermentation products during growth with PYG were acetate and butyrate. Based on phylogenetic, phenotypic and chemotaxonomic information, strain NE08V was identified as representing a novel species of the genus Clostridium, for which the name Clostridium amazonense sp. nov. is proposed. The type strain is NE08V T (DSM 23598 T = CCUG 59712 T ). Highlights: A novel Clostridium species was isolated from a remote Amazonian community in Peru. Novel organisms will provide insights into differences between remote and westernized communities. Interrelationships between taxa will provide functional aspects of such ecosystems.